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17-hydroxycorticosteroid urine test

The 17-hydroxycorticosteroid test measures the level of 17-OHCS in the urine.

How the Test is Performed

A 24-hour urine sample is needed. You will need to collect your urine over 24 hours. Your health care provider will tell you how to do this. Follow instructions exactly so that the results are accurate.

How to Prepare for the Test

The provider will instruct you, if necessary, to stop medicines that may interfere with the test. These may include:

  • Birth control pills that contain estrogen
  • Certain antibiotics
  • Glucocorticoids

How the Test will Feel

The test involves only normal urination. There is no discomfort.

Why the Test is Performed

17-hydroxycorticosteroid (17-OHCS) is a product formed when the liver and other body tissues break down the steroid hormone, cortisol.

This test can help determine if the body is producing too much of the hormone, cortisol. The test may be used to diagnose Cushing syndrome. This is a disorder that occurs when the body has a high level of cortisol.

Normal Results

Normal values:

  • Male: 4 to 14 mg per 24 hours
  • Female: 2 to 12 mg per 24 hours

Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your provider about the meaning of your specific test results.

What Abnormal Results Mean

A higher than normal level of 17-OHCS may indicate:

A lower than normal level of 17-OHCS may indicate:


There are no risks with this test.

Alternative Names

17-OH corticosteroids; 17-OHCS


Chernecky CC, Berger BJ. 17-hydroxycorticosteroid - urine. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. Philadelphia, PA: Elsevier Saunders; 2013:659-660.

Juszczak A, Morris DG, Grossman AB, Nieman LK. Cushing's syndrome. In: Jameson JL, De Groot LJ, de Kretser DM, et al, eds. Endocrinology: Adult and Pediatric. 7th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 13.

Review Date 10/28/2015

Updated by: Brent Wisse, MD, Associate Professor of Medicine, Division of Metabolism, Endocrinology & Nutrition, University of Washington School of Medicine, Seattle, WA. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.