Immunoelectrophoresis - urine

A clean-catch urine sample is needed. The clean-catch method is used to prevent germs from the penis or vagina from getting into a urine sample. To collect your urine, your health care provider may give you a special clean-catch kit that contains a cleansing solution and sterile wipes. Follow instructions exactly.

After you provide a urine sample, it is sent to the lab. There, a lab specialist will place the urine sample on special paper and apply an electric current. The various proteins move and form visible bands, which reveal the amounts of each protein.

Your provider may ask you to collect the first morning urine, which is the most concentrated.

If you are taking the collection from an infant, you may need extra collection bags.

The test involves only normal urination, and there is no discomfort.

This test is used to measure the amounts of various immunoglobulins in urine. Most often, it is done after a large amount of protein is found in the urine.

Normally there is no protein, or only a small amount of protein in the urine. When there is protein in the urine, it normally consists of mainly albumin.

Normal value ranges may vary slightly among different laboratories. Talk to your provider about the meaning of your specific test results.

Immunoglobulin in the urine can result from:

• Amyloidosis (buildup of abnormal proteins in tissues and organs)
• Leukemia (cancer of the bone marrow)
• Waldenström macroglobulinemia (a type of lymphoma)
• Lymphoma (cancer of the lymph tissue)
• Monoclonal gammopathy of unknown significance (MGUS, a type of blood disorder)
• Multiple myeloma (a type of blood cancer)
• Other cancers
• Infection
• Kidney disorders such as IgA nephropathy or IgM nephropathy

Some people have monoclonal immunoglobulins, but do not have cancer. This is called monoclonal gammopathy of unknown significance, or MGUS.

There are no risks with this test.

Immunoglobulin electrophoresis - urine; Gamma globulin electrophoresis - urine; Urine immunoglobulin electrophoresis; IEP - urine

Gertz MA, Dispenzieri A. Amyloidosis. In: Goldman L, Cooney KA, eds. Goldman-Cecil Medicine. 27th ed. Philadelphia, PA: Elsevier; 2024:chap 174.

McPherson RA. Specific proteins. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 20.

Rajkumar SV, Dispenzieri A. Multiple myeloma and related disorders. In: Niederhuber JE, Armitage JO, Kastan MB, Doroshow JH, Tepper JE, eds. Abeloff's Clinical Oncology. 6th ed. Philadelphia, PA: Elsevier; 2020:chap 101.

Updated by: Todd Gersten, MD, Hematology/Oncology, Florida Cancer Specialists & Research Institute, Wellington, FL. Review provided by VeriMed Healthcare Network. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.