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Culdocentesis

Culdocentesis is a procedure that checks for abnormal fluid in the space just behind the vagina. This area is called the cul-de-sac.

How the Test is Performed

First, you will have a pelvic exam. Then, the health care provider will hold the cervix with an instrument and lift it slightly.

A long, thin needle is inserted through the wall of the vagina (just below the uterus). A sample is taken of any fluid found in the space. The needle is pulled out.

How to Prepare for the Test

You may be asked to walk or sit for a short time before the test is done.

How the Test will Feel

You may have an uncomfortable, cramping feeling. You will feel a brief, sharp pain as the needle is inserted.

Why the Test is Performed

This procedure is rarely done today because a transvaginal ultrasound can show fluid behind the uterus.

It may be done when:

  • You have pain in the lower abdomen and pelvis, and other tests suggest there is fluid in the area.
  • You may have a ruptured ectopic pregnancy or ovarian cyst.

Normal Results

No fluid in the cul-de-sac, or a very small amount of clear fluid, is normal.

What Abnormal Results Mean

Fluid may still be present, even if not seen with this test. You may need other tests.

A sample of fluid may be taken and tested for infection.

If blood is found in the fluid sample, you may need emergency surgery.

Risks

Risks include puncturing the uterine or bowel wall.

Considerations

You may need someone to take you home if you were given medicines to relax.

References

Kho RM, Lobo RA. Ectopic pregnancy: etiology, pathology, diagnosis, management, fertility prognosis. In: Lobo RA, Gershenson DM, Lentz GM, Valea FA, eds. Comprehensive Gynecology. 7th ed. Philadelphia, PA: Elsevier; 2017:chap 17.

Braen GR, Kiel J. Gynecologic procedures. In: Roberts JR, Custalow CB, Thomsen, TW, eds. Roberts and Hedges' Clinical Procedures in Emergency Medicine and Acute Care. 7th ed. Philadelphia, PA: Elsevier; 2019:chap 57.

Review Date 1/14/2018

Updated by: John D. Jacobson, MD, Professor of Obstetrics and Gynecology, Loma Linda University School of Medicine, Loma Linda Center for Fertility, Loma Linda, CA. Also reviewed by David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.