Skip navigation

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

URL of this page: //medlineplus.gov/ency/imagepages/9750.htm

Pleural culture

Pleural culture

Overview

A sample of pleural fluid is withdrawn by a needle and sent to the laboratory for testing. The sample pleural fluid is placed on culture plates containing growth media. When colonies of microorganisms have reached sufficient size, a series of biochemical tests can be performed to identify the presence of specific organisms. The test is performed when infection of the pleural space is suspected or when an abnormal collection of pleural fluid is noticed by chest X-ray.

Review Date 12/31/2023

Updated by: Jatin M. Vyas, MD, PhD, Associate Professor in Medicine, Harvard Medical School; Associate in Medicine, Division of Infectious Disease, Department of Medicine, Massachusetts General Hospital, Boston, MA. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.